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Tracking is performed by labeling individual atoms or groups of atoms of interest, and solving, in addition to the coupled ordinary differential equations describing the time evolution of species concentrations, appropriate transfer equations providing the concentration of tracked atoms at each possible location on all chemical species involved.
Metronidazole (Mtz) was added at various concentrations for quantitative scoring of susceptibility and resistance at concentrations appropriate for the strains being tested, as described [20], [29].
Primary antibodies, anti-GFP, anti-Troponin T-C (C-19), anti-Actin (C-11) (all from Santa Cruz Biotechnology, Inc ., or anti-Sarcomeric Myosin, MF20 (Developmental Studies Hybridoma Bank), were diluted in blocking solution to concentrations appropriate to the specific antibody and added to cells.
However, the NEM concentrations appropriate to provide a gradient depletion of protein thiols and total thiols were found to be too high for depleting nonprotein thiols.
Special attention is paid to the main challenges when analyzing these toxic food contaminants in urine, i.e., very low analyte concentrations, appropriate sample preparation, matrix effects, and a lack of authentic, NMR-confirmed calibrants and reference materials.
In addition to these differences, other characteristics unique to the paediatric population include the lack of commercially available dosage forms and concentrations appropriate for paediatric patients and the lack of published research on the pharmacokinetics and clinical use of new drugs [ 9].
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Then, the obtained solution was diluted to adjust the Au concentration to a concentration appropriate to the ICP measurement.
From the initial concentration, appropriate decimal dilutions were prepared and aliquots were plated in duplicates on various media.
After experimental establishment of sample concentration appropriate for size measurement, the electrolyte solution of the desired ionic strength was added to 1 mL of diluted sample in a measurement cell and mixed by stirring.
To determine the GC7 concentration appropriate for coadministration with doxorubicin, we tested the effect of a series of GC7 concentrations on bladder cell viability using the CCK8 assay.
Sample libraries were diluted and applied to an Illumina paired end flow cell at a concentration appropriate to generate about 180 million reads per lane.
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