The fractional kill hypothesis states that a defined chemotherapy concentration applied for a defined period of time will kill a constant fraction of cells in a population regardless of tumour size, relative to cell sensitivity to treatment.
To avoid background staining due to the high content of mucus in colon and colorectal cancer tissue, slides were, in addition, pre-incubated for 30 min with 2% serum corresponding to the species of the secondary antibody before the addition of primary antibodies, which have been used at half the concentration applied for staining of non-colorectal tissue.
End-of-assay medium was collected from the assays, protein levels measured and equal concentrations applied to ELISA plates for MMP-1, MMP-2 and MMP-9 (Amersham, Buckinghamshire, UK).
Therefore, evaporation losses without cells had to be re-estimated after 3 h incubation for the concentrations applied to hepatocytes in order to prevent concentration-related mismatches.
Figure 1 demonstrates the beating rate in beats per minute and rate change in relation to control for all concentrations applied.
This enforcement was significant for 100 μg/ml VAE-M, compared to 0 μg/ml VAE-M, for all doxorubicin concentrations applied (p < 0.05).
Concentrations of heavy metal mixtures were calculated by dividing the growth IC50 of each heavy metal by 4 and two higher and two lower concentrations were applied for screening bioassay.
To reduce the possibility of nonspecific effects of the drug treatments, inhibitors were used at low concentrations and applied for a short period of time (5 10 min).
However, the inhibitors were used at high concentrations and applied for long time frames in these experiments, which makes deciphering of the role of actin filament dynamics in plant mitochondrial movements impossible and may cause nonspecific effects of drug treatments.
Extract concentrations were applied for different times: 2 and 5 days.
