Nicholson et al. recently found this locus to be associated with dimethylamine concentrations in plasma, also using NMR spectrometry [ 6].
Besides the full-strength standard top agar concentration (0.8%), 2/3- (0.53%) and 1/3-strength (0.27%) of concentrations were also used to determine the effect of top agar concentration on phage diffusion.
Additionally, cells were treated upon LPS incubation with two different DHEA concentrations (10-5 M or 10-8 M; Sigma, Deisenhofen, Germany); these concentrations were also used as single stimuli to determine DHEA specific effects.
Six concentrations were also used for the DMSO extract: 0.016, 0.08, 0.40, 2, 10 and 50 mg per plate equivalent.
Stratification metrics and river nutrient concentrations were also used to develop a regression model for hypoxic area (y, km), as described in Section 2.7.
The standardized concentrations were also used to calculate the cutpoints for the quintiles and the "low," "moderate," and "high" categories of serum 25(OH D.
Phosphate and citrate buffers and higher salt concentrations were also used for a limited set of spectra and found to give identical results for a given pH.
In addition, evidence of poor replication between analytical duplicates, and extreme THAA concentrations, were also used to screen data (Section 2.4.4; Electronic Annex, EA).
Natural log-transformed concentrations were also used as independent variables in regression analysis to minimize the potential influence of extreme values on the regression coefficients.
Since previous studies defined a person who could not recognize these concentrations of solution as having sweet-taste, salt-taste, sour-taste and bitter-taste hyposensitivity [ 7, 8], the same concentrations were also used in this study.
