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The precipitated proteins from N2-4 and N3-8 isolates were filtered through 0.2 µm membranes and the concentrations adjusted to 3.0 mg/ml and used to determine the MIC and MBC by micro-broth dilution (Hoelzer et al. 2011).
We showed that hF.IX expression in the plasma could be expressed to therapeutically significant concentrations, adjusted to different set levels by varying the tetracycline dose, rapidly turned off and on, and completely recovered after each treatment cycle.
Five lowland tropical forest soils with contrasting properties (pH 4.4 6.1, total P 86 429 mg P kg− 1) were extracted with 2 mM citric acid (i.e., 10 μmol g− 1, approximating rhizosphere concentrations) adjusted to soil pH in a 4 1 solution to soil ratio for 1 h.
Fragments A to E were screened at concentrations adjusted to the affinity of each fragment in duplicate.
DNA samples were quantified using the Quant-IT kit (Qiagen, USA) and concentrations adjusted to 10 ng/μl using nuclease-free water (Ambicon, USA).
The murine peritoneal macrophages and the J774 cells suspension with concentrations adjusted to 1 × 10 cells/mL were seeded at a density of 2 × 10 cells/well in 96-well plates (100 μL per well) and cultured for 24 h.
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Chloride serum concentration adjusted to blood sodium level, acid base status, routine laboratory, and clinical variables were measured at admission.
For Cys concentration adjusted to 5 μM, an excellent limit of detection of 23 nM was achieved.
The seeding cells were detached from the culture flasks with co-enzymes and the cellular concentration adjusted to 2 × 106/mL with DMEM containing 10% FBS.
Finally, the number of total cells was quantified by flow cytometry and the concentration adjusted to 1 × 107 cells/mL in TSB0.25 %G with or without antibiotics.
After extensive dialysis to remove nonionic surfactant and preservative, the vaccine was diluted into 1xPBS with protein concentration adjusted to 500 ug/ml.
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