p53 protein (0.5 µg) was diluted in 50 µl PBS, 0.05% (v/v) Tween-20, 0.2% (w/v) BSA and (in increasing concentration) added into NTD125/CHIP/BSA coated wells.
Each extract was diluted to the desired concentration, added into the cell culture, and then incubated for 72 hours.
Agonists, glutamate or DHPG, were 1 5 serially diluted into 11 point concentrations, added to cells, and incubated for 2 min. The agonist-mediated calcium response was calculated by subtracting the basal florescent peak before agonist addition from the maximal peak elicited by agonist.
The transmission spectrums of nanoCA were recorded by the optical system, when 30 μL analyte solution at different concentrations was added into different cavities.
To assess the effect of liposomes on cell apoptosis, liposomes with different lipid concentrations were added into the cells and incubated for 4 h.
Briefly, 0.5 mL of Fe3O4 microsphere stock solution (with different concentrations) was added into 10 mL aqueous solution of methylene blue (2 μg/mL) at pH 5.0.
Subsequently, 100 μL of a AgNP solution at 1.0, 500, 10, 50, and 100 μg/mL concentrations were added into 3 mm diameter wells, cut out in the centre of the plate, and incubated at 37 °C for 24 h.
In another plate, the suspensions of AuNR@PAA/CaP yolk shell NPs with different concentrations were added into HeLa cells with the 808-nm NIR laser irradiation for 5 min.
For the adsorption tests, 10 mL of organic dyes (RhB, MB, MO, and Rh6G) in aqueous solution with different concentrations were added into the quartz tube with 5 mg of the as-prepared samples.
Inhibitors with certain concentrations were added into the dispersion and stirred for 20 min, after aged for 24 h, and then the size distribution of the particles was measured by LS-13320 laser particle size analyzer based on the light scattering principle (Beckman Coulter, Inc., USA) using equipment operating procedure under the pump speed of 40%.
