When the concentration response effect of histamine on stretch-induced ATP release was determined, it was seen to occur with an IC50 of 0.24 μM (0.19 2.8 μM), n = 6).
For our work and suggestions contained in later section of this article, that basis includes: … hazard identification for the effect based on all available non-human information; —the establishment of the quantitative dose (concentration)—response (effect) relationship.
When the concentration response effect of ADP induced ATP release was determined it was seen to only occur at high concentrations of ADP) with an EC50 of 1.8 μM determined (n = 5).
… When it is not possible to establish the quantitative dose (concentration)–response (effect) relationship, then this should be justified and a semiquantitative or qualitative analysis shall be included… Unfortunately, the devil is in the details, and these do not appear to be specified.
Furthermore, the experimental groups showed similar saccharin self-administration during the concentration-response test (group effect and group x concentration interaction: n.s)., with the response of both groups following a bell-shaped curve (concentration effect: F 7, 98) = 39.7, p<0.0001) (Figure 4D).
Based on these concentration-response data, mixture effects were predicted by applying the model of concentration addition.
To determine the concentration-response curve, the effects of doses of 0 μg/mL, 10 μg/mL, 50 μg/mL, and 100 μg/mL of propolis and propolis fractions were determined by the release of superoxide anions by phagocytes as described in Section 2\tmspace +\thinmuskip {.1667em}2.9.
For each concentration-response curve, the maximal effect (Rmax) and the concentration of agonist that produced 50% of the maximal response (log EC50) were calculated using non-linear regression analysis (GraphPad Prism, GraphPad Software, Inc., San Diego, CA).
Additional steps include sensitivity analysis, such as applying multiple climate change scenarios or concentration-response functions for health effects.
Concentration-response curves for the effects of flupirtine on GABAA receptors were collected in the presence and absence of 5 mM penicillin.
