This effect appeared to be additive to the glucocorticoid-mediated effect, because stimulation with sub-saturating concentrations of fludrocortisol (0.03 nM) in stripped serum containing media resulted in higher MR activation than in serum free media (Fig. 3D).
Si G5, at concentrations between 100 to 750 ng/mL, completely reversed this H2O2- mediated effect, and reduced caspase-8 to lower than basal levels.
Conclusions: Clinically relevant concentrations of DMSO impair platelet activation by a thromboxane A2-dependent, COX-1-mediated effect.
The effect was dose responsive, although raising the concentration to 1000 nM caused no further increases; this would concur with the saturation of a receptor-mediated effect, as reported in other tissues (19, 38, 39).
Low concentrations of SA or 10H2DA significantly induced mineralization, which was suppressed by the addition of ICI182780, indicating an ER-mediated effect.
αβ (mediated effect) product-of-coefficient estimate.
Finally, an experiment that used a scrambled RDG peptide at a concentration of 100 μ M had no significant effect on the rate of migration, showing the specificity of the peptide-mediated effect.
Experimentalists thus could greatly contribute to the further accumulation of informative benchmark data by generating dose-response data at or near half-maximal response levels, expressing antibody-mediated effects as apparent concentration-dependent changes in median effective doses of particular causative agents wherever possible.
Together, these data indicate that: 1) MES and SUL are responsible for a significant part of the antidopaminergic effects attributed to THD; 2) THD should produce less cholinergic activation than other neuroleptics; and 3) that nonreceptor-mediated effects at high THD concentrations may mask effects due to receptor blockade.
These different actions were demonstrated to depend on the concentration of K201 and concentrations up to 1 μM K201 are used to ensure RYR-mediated effects [ 12].
