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Bisulfite DNA quality and concentration were assessed using the NanoDrop and, if required, samples were concentrated to approximately 50 ng/ul using a SpeedVac (Thermo Electron Corporation, Waltham, MA, USA).
Variations between different chilling stresses and foliar ASA concentration were assessed by univariate analyses followed by post hoc analysis.
The distributions of temperature, relative humidity (RH), and CO2 concentration were assessed throughout the test site for four scenarios.
The impact of pH and loading buffer salt concentration were assessed using robotically operated 20 μL packed pipette tips.
Parameters such as cooling rate, plunge temperature and cryoprotective agent concentration were assessed using a custom built device based on Pye's freezer idea.
The effects of process and material variables on the permeate flux decay, steady-state permeate flux J∞ and the permeate surfactant concentration were assessed.
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Its critical aggregation concentration was assessed by fluorescence probe technique.
In binary systems, the deviation from linear dependence of Q on solute concentration is assessed.
The effect of reaction temperature and initiator (K2S2O8) concentration was assessed on these general observations.
The protein concentration was assessed according to Bradford.
Protein concentration was assessed using the BCA assay (Pierce).
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