Briefly, pol V and RecA proteins at various known concentrations were resolved on an SDS-PAGE gel (10%) as standards and gel band intensities were quantified using IMAGEQUANT software.
After addition of 2x Laemlli buffer, equal concentration of protein was resolved on 10% SDS-PAGE followed by immunoblotting with anti-Hsp70 and anti-actin antibodies on nitrocellulose membrane.
The reaction was stopped by adding Laemmli buffer to 1 × concentration, and protein was resolved by SDS-PAGE and visualised by staining with CBB.
A final volume of SDS-loading buffer (1 M Tris-HCl, pH 6.8, 10% SDS, 0.5% β-mercaptoethanol, 0.1% bromophenol blue) was then added to the washed pellet (corresponding to 100x concentration) and this mix was resolved on 15% SDS PAGE after the samples had been heated for 10 min at 75°C.
Briefly, a standard concentration of purified Stx2 (3 μL/10 μL) was resolved into its A and B subunits by SDS-PAGE.
In the LEAD trials, a minor hypoglycemic event was defined as a plasma glucose concentration of <56 mg/dL that was resolved with self-treatment, and a major hypoglycemic event was defined as a hypoglycemic event requiring third party assistance.
This problem was resolved using excessive concentrations of proteinase K and SDS in the lysis solution and an optimized buffer for proteinase K digestion.
The melphalan was resolved in ethanol resulting in a final ethanol concentration of 0.06% in the medium.
RNA was resolved in RNase free water (Roth), and concentrations were determined using a NanoDrop 1000 Spectrophotometer (Peqlab).
A quantity of 60 mg was resolved in 1 ml of distilled water, resulting in a concentration of 0.05 M.
