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In this test 2 g dry matter soil for each concentration was mixed with 200 mL of ultra-high-quality water and homogenised for 3 min at 9,600 rpm with a homogeniser.
For the probe preparation, 50 mL solution at each concentration was mixed with 2 mg each of hematin and luminol.
900 μL of each concentration was mixed with 100 μL of sterile nutrient broth containing 10 CFU bacteria, (obtained from a McFarland turbidity standard no. 0.5).
AMS with adsorbed BSA with equilibrium protein concentration was mixed with 5 mL fresh PB (pH 5.5; 10 mM) at room temperature.
A series of concentrations (0 - 400 μg/ml) of PKC extract was prepared and 60 μl of PKC extract in different concentration was mixed with 10 mM sodium nitoprusside in phosphate buffered saline (PBS).
Before microseeding, 2 μl of protein solution at 22 mg/ml concentration was mixed with an equal volume of reservoir solution containing 64% saturated ammonium sulfate, 0.1 M Hepes (pH 7.5) and 4% methyl-2,4-pentanediol, and equilibrated overnight.
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For this purpose, antibiotic solutions having the same concentration were mixed with equal volumes of 0.1, 0.5, and 1.0 mM gold solutions in 2 mM K2CO3before being added to the wells.
Three microlitres of the protein solution at 3 mg/ml concentration were mixed with an equal volume of the reservoir solution containing the precipitant in 200 mM NaCl, 2 mM β-mercaptoethanol, 0.1% sodium azide, 100 mM Tris-HCl, pH 3.0 9.5.
Meanwhile, 2.5 ml HSA solutions of varied concentration were mixed with 2.5 ml of the above PNBN-T solution.
Briefly, One ml methanolic extract of each of plant at different concentration were mixed with 3 ml 0.1 mM solution of 2,2-diphenyl-1-picrylhydrazil (DPPH) in methanol.
For quantification of cytochrome c in whole worm extract and in mitochondrial preparations, frozen samples with known protein concentration were mixed with Laemmli buffer, heated at 99°C for 5 min and equal amounts of protein were loaded on gels.
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