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APN (5 μg/mL: this concentration was decided, based on the previous publications 17, 18 or phosphate buffer saline (PBS) for an additional 18 h).
Once the thrombin concentration was decided, the purified protein was mixed with proper amounts of thrombin and dialyzed in low salt buffer (20 mM Tris, 100 mM NaCl, pH7.5) at 4°C for 16 hours.
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The 3 concentrations were decided that the difference in between two consecutive levels is the same.
PQ treatment concentrations were decided upon in preliminary life history assays; PQ levels that dramatically reduced or prevented nematode development to maturity under otherwise standard conditions were avoided.
Accordingly, in this work to improve the corrosion resistance of stainless steel in NaCl solutions with different concentrations, it was decided to make use of the useful properties of manganese oxide on SS316L.
A concentration of 500 ppm was decided to be the ionic liquid concentration used for flooding runs.
The concentration (0.03 % w/w) was decided on the basis of colorimetric data obtained for laboratory stone samples treated with differently loaded nanocomposites [14].
As flavonoid and anthraquinone colorants differed substantially as well as they needed different extraction temperature and acid concentrations in the solvent, it was decided to choose average values of the optimized parameters, which would be the most "universal" for all dyes.
It was decided that this concentration should be lowered to about 2 mg P l−1 to maintain a safe nitrogen : phosporus (N : P) ratio of about 10.
In the second step, among the 360 triplets, the exhaustive concentration range for any given triplet was decided by the outcome of the first step.
It was decided to limit the data gathering for volumetric concentrations of nanoparticles (F) up to 10% (focus group).
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