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Check dsRNA concentration using a spectrophotometer and dilute accordingly.
During the measurement, the electrolytes were stirred to ensure uniform RM and oxygen species concentration using a small size magnetic bar in the cuvette.
Libraries were quantified and normalized using PicoGreen to ensure equal concentration using a Perkin Elmer MiniJanus instrument and pooled by equal volume on the Agilent Bravo instrument.
Prior to sequencing, all amplicons were assessed for fragment size distribution and DNA concentration using a Bioanalyser 2100 (Agilent Technologies, USA).
The instrument determines volume (or mass) concentration using a volume conversion factor Cv.
The release was measured as a function of current density and NaCl concentration using a novel iontophoretic cell.
An adaptive method is developed to control the end-tidal isoflurane concentration using a low-flow method.
After two hours of incubation, gas samples were analyzed every hour for the remaining five hours to measure N2O concentration using a gas chromatograph (Micro GC R3000, SRA Instrument, Marcy L'Etoile, France).
Excess unconjugated PEAS was removed by buffer exchange (100-fold) with octamer dilution buffer (5 M Tris-HCl pH 8, 0.5 mM EDTA, 1 M NaCl) and subsequent concentration using a 30 KDa cutoff Amicon ultrafilter column.
The key idea here is to replace the effect of optical concentration using a different method.
Samples of the effluent were collected periodically and analyzed for the remaining nitrate anion concentration using a spectrophotometer.
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