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The infectious agent was definitively identified as vancomycin-intermediate, daptomycin-nonsusceptible, rifampicin-resistant ST72-MRSA-IV based on culture results and minimum inhibitory concentration testing.
Concentration testing was also done on GLA-SE by HPLC [20] and found to be consistent through the formulation procedure (data not shown).
Blood alcohol concentration testing was only performed in 18 cases (5.1%), 16 of these were positive.
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The highest concentration tested, about 24percentt in OFF! Deep Woods, lasted five hours on average.
IC90 values were the lowest concentration tested that resulted in ≥90% inhibition of growth.
ROS assay also revealed that at concentration tested, PtNPs fails to induce ROS in E. coli (Supplementary Fig. 13).
The Ca2+-dependency of the rate of trans binding was evident at every protein concentration tested (Fig. 4B).
When one skip was observed, the IC90 value was consistently interpreted as the lowest concentration tested that resulted in ≥90% inhibition of growth.
The time to reach the half-maximal signal was 9.2 ± 0.3 and 12.0 ± 0.4 min, at 37 °C and 25 °C, respectively when the concentration tested was 400 nM.
It is dose-dependent and ATP, for example, showed a strong inhibitory activity even at the lowest concentration tested (10 μM).
The inoculum was split and spread onto four large (14 cm diameter) MHA-SA agar plates for each antimicrobial concentration tested in a two-fold dilution series.
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