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After 24 h of treatment, the media was aspirated out and 500 μl of 1X-PBS was added followed by addition of fluorogenic substrates (20 μM final concentration) specific for the chymotrypsin-like (Ch-L), trypsin-like (T-L) and caspase-like (Cp-L) activities of the 20S proteasome.
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The content of pure alcohol was calculated according to the following concentrations specific for Spain: 5% for beer; 12 percent for wine; and 40percentt for hard liquors.
Wortmannin abolishes plasma membrane PtdIns4 P staining at the high (10 μM), but not lower (300 nM), concentrations specific for PI3K.
The finding of increased sulfur colloid concentration is specific for FNH[ 100 ].
The assay measures total liraglutide plasma concentration and is specific for intact liraglutide.
For western blot analysis, cellular extracts were separated on SDS-polyacrylamide gels with a concentration of acrylamide specific for the proteins studied.
Again this is consistent with our data, which showed that this serum Co concentration was 88% specific for detecting abnormal wear.
We examined sequentially the serum concentration of C2C, specific for the destruction of type II collagen by MMP-1, MMP-8, and MMP-13.
Increased intracellular glucose concentration triggers processes specific for oxidative stress and/or pro-inflammatory state, in particular an increase in the secretion of mitochondrial reactive oxygen species (ROS) [ 5- 8].
It is possible that the degeneracy of the CoCoMo primers could be too high, which would reduce the concentration of primers specific for a particular target sequence and decrease the sensitivity of the assay.
Our results reveal for the first time that the induction of colitis or ileitis in mice is associated with marked increases in the luminal concentrations of PAMPs specific for TLR2, TLR4 and/or TLR5.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com