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When the concentration response effect of histamine on stretch-induced ATP release was determined, it was seen to occur with an IC50 of 0.24 μM (0.19 2.8 μM), n = 6).
For our work and suggestions contained in later section of this article, that basis includes: … hazard identification for the effect based on all available non-human information; —the establishment of the quantitative dose (concentration)—response (effect) relationship.
When the concentration response effect of ADP induced ATP release was determined it was seen to only occur at high concentrations of ADP) with an EC50 of 1.8 μM determined (n = 5).
… When it is not possible to establish the quantitative dose (concentration)–response (effect) relationship, then this should be justified and a semiquantitative or qualitative analysis shall be included… Unfortunately, the devil is in the details, and these do not appear to be specified.
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In the same manner, we also tested if the concentration-response effect differed between the isoforms.
In this study, statistical estimates of effect concentrations lower than the corresponding NOECs were derived by regression analysis of concentration response data, down to effect levels of 1%.
For the concentration response, a bell-shaped curve (concentration effect: F 7, 126) = 31.8, p<0.0001) was observed, with no difference between groups (group effect and group x concentration interaction: n.s).s
Importantly, there is a dose response effect of the concentration of DA on the modulation of the glutamate-induced calcium response.
BsAb titration studies demonstrated a clear dose response effect of BsAb concentration on target cell protein synthesis inhibition and cell proliferation.
This level closely matches current regulated ambient levels, although the toxicodynamics of this phenomenon requires a study with greater precision and better characterization of concentration response and the temporal nature of effects in order to extrapolate with confidence to potential environmental effects.
Our studies offer a measure of the magnitude of this error that in turn contributes to the variability of the biological effects expressed in concentration response relationships and estimates of functional potency.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com