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All stool specimens were concentrated by using the formalin-ethyl acetate concentration procedure routinely used to examine ova and parasites in stool specimens (2 ).
Their antifungal activities against four kinds of phytopathogens were evaluated using the radial growth assay and minimum inhibitory concentration procedure.
The solution methodology complements a heuristic concentration procedure, where sub-problems with reduced sets of decision variables are iteratively solved to optimality.
A column of cation-exchange resin in Ag+ form and an anion-exchange column in series were used in the concentration procedure.
The digestion and concentration procedure took in total 3 h.
We also implemented a straightforward concentration procedure based on Mustang Q anion exchange membrane chromatography.
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This method also bypasses tedious PCR purification and concentration procedures, and it facilitates large-scale SNP studies by using a method that is highly sensitive, simple, labor-saving, and potentially automatable.
To improve sensitivity, multiple specimens and concentration procedures, as well as a variety of staining methods, are needed to achieve ample sensitivity [ 13, 14].
The ultraviolet absorption spectrum of CdBP also was variable, with 250/280 nm ratios ranging from 17:1 immediately after ion-exchange chromatography to 2 1 following concentration procedures.
These protocols are easy to implement in a standard laboratory setting and allow high-titer LV vector production without the need for multiple tissue culture dishes or flasks or ultracentrifugation-based concentration procedures.
The selectivity of pre-concentration procedure appeared the major concern for environmental samples analysis.
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