To estimate the original bacteriophage concentration, plates with one to 100 distinguishable homogenous plaques were counted depending on the phage plaque size.
Replicates of 10 were performed for each concentration, plates were incubated overnight at 37°C, and colonies were counted the following morning.
To optimize oxacillin concentration, plates containing the agar base supplemented with twofold dilutions of oxacillin (0.062 0.5 µg/ml) with or without 2 % NaCl were tested using 15 clinical MRSP isolates belonging to 10 multi-locus sequence types, and 15 randomly selected clinical S. pseudintermedius isolates susceptible to methicillin.
After 24 48 h, a mixture of growth from the highest concentration plate with multiple colonies was used to start a new overnight culture at the same antimicrobial concentration.
Polymer solution concentration, plate size, and applied voltage were all shown to have varying effects on maximum fiber length, fiber diameter, and fiber uniformity.
The nickel/nickel oxide thin films were deposited via electrolysis and the quality was evaluated via the effect of the sulfate concentration, plating temperature, plating solution pH, and heat treatment.
Effects of the sodium acetate (NaCH3COO, denoted as NaAcO) concentration, plating temperature, and oxide loading on the pseudocapacitive characteristics of hydrous ruthenium oxide (denoted as RuO2·xH2O) films anodically plated from aqueous RuCl3·xH2O media were systematically investigated in this work.
The cells from a few hundred colonies on a low antibiotic concentration plate were then pooled, and again plated on LB only, for titering cell concentration, as well as on plates with varying antibiotic concentrations, to determine survival rates, reversion rates, and to increase antibiotic resistance.
Samples were assessed for total AO activity using the peroxyl radical scavenging capacity assay where each sample was diluted to 5 descending concentrations, plated in triplicate.
In both cases, additional time was required for a scientist to plan the experiment (e.g., identify antimicrobial test concentrations, plate layout, and appropriate control experiments) and prepare solutions (e.g., sterile media, overnight cultures, antimicrobial stock solution).
Single-concentration plate assays were used to assess resistance to ampicillin, chloramphenicol, erythromycin, gentamicin, tetracyline and vancomycin (see Table 1 and Additional file 2).
