Seedlings were supplied with 30 ml liquid PNM media containing the two different Pi concentrations, once a week.
A 50-mL milk sample was taken to determine the milk fat and milk protein concentrations once a week (MilkoScanTM FT1, FOSS, Denmark).
The same percentage of spread cells (i.e. cells that switched from round to flat with filopodia and maintained this spread form) was observed independent of local concentration once local concentration exceeded a minimum threshold of 4 dots/cluster (and with a constant global concentration of 50 RGD/μm) [ 53].
Or rather, zero albums consumed voluntarily with anything approaching concentration: once I was driving with a friend and his girlfriend, and she put the first album on the car stereo through her iPhone, and I liked what I heard, but can't remember a thing.
This 2 ½-hour film, which is described by Mr. Tiravanija as "not a documentary and not a narrative" but "more of a portraiture," rewards concentration once you adjust to its glacial pace and its radically minimalist aesthetic.
T7 dependent expression was induced by addition of IPTG (100 µg/ml final concentration) once cell cultures had reached an OD600 of 0.4 0.5.
"Also, a lack of concentration once can cost you games and you lose games because of that.
They usually contain preservatives, stabilisers and suspending agents to ensure they remain reasonably stable and at an evenly mixed concentration once water is added.
All major ions of interest (e.g. As, Ca, Se, SO4) displayed a similar temporal leaching pattern, with much higher concentrations present in initial leachates and then a rapid drop in concentration once several pore volumes (6 10 leach cycles) had eluted (Daniels et al. 2014).
However, they can adjust the intensity of their antipredator responses to changes in relative concentration once they have learned that the predator odour represents a threat.
