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The cleaving process caused a frequency change of QCM to give a signal related to the concentration of type IV collagenase.
Production of type 1 and 3 collagens were determined by measuring the concentration of type 1 and 3 collagens N-terminal propeptide (PINP and PIIINP) from cell culture media.
Type I collagen (5 mg/ml, Sigma-Aldrich), 10x DMEM, and cell suspension were mixed on ice in a volume ratio of 6∶2.5∶1.5 (final concentration of type I collagen, 3 mg/ml; final cell density, 2×105 per ml).
For the examination of kidney, this was carried out by means of a modified Prionics check protocol (Prionics AG, Switzerland), with 1 g of kidney being first incubated in TBS buffer (10 mM Tris HCl, 133 mM NaCl, pH 7.4) containing 1,5 mM CaCl2 and 2.5 mg/ml (final concentration) of type XI collagenase 1.6 U/mg (Sigma, USA) for 2 h at 37°C.
Perception of sufficient concentration of type 1 cytokine induces tumor necrosis factor alpha (TNF‐α) secretion in microglia.
The mean free testosterone concentration of type 2 diabetic patients in our study was 0.296 nmol/l.
Similar(42)
In this study, the pre- and post-HD plasma concentrations of type IV collagenases and their inhibitors in HD patients were examined.
Using Kernel density estimation, it was determined that areas of high deforestation density were related to high concentrations of type I and IV farms, whereas agriculture expansion was related to types I and II.
The amount of cumulative hydrocarbon generation can strongly depend on compositions and concentrations of Type II III and Type III kerogen.
In our experiments, we did not detect type 1 IFNs in the culture supernatants of the lymphoid tissue ex vivo although, we readily detected it in supernatants from the tissues spiked with various concentrations of type 1 IFNs.
Coating with different concentrations of type IV collagen or gelatin resulted in similar switches.
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