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The protein concentration of tissue homogenates was determined using the BSA method as described previously [17].
In this condition, the extraordinarily high concentration of tissue factor at the placenta site may constitute a principle of treatment as it activates coagulation very effectively.
It detects the hemodynamic changes in tissues via noninvasive measurement of changes in the concentration of tissue chromophores such as oxyhemoglobin (O2Hb) and deoxyhemoglobin (HHb).
Approximately, a known protein concentration of tissue supernatant was added to a reaction mixture containing 0.1 mM EDTA (200 μl), 0.12 mM riboflavin (50 μl), and 0.6 M phosphate buffer (pH 7.8) in a final volume of 3 ml.
In the working hypothesis we assumed that the procoagulant activity of microparticles (MPs) is associated with the concentration of tissue factor (TF) and its inhibitor (TFPI), and that these three components together affect fetal hemostasis.
Because gastrin is a potent secretagogue linked to acid secretion, this study was designed to investigate, in pigs with or without GUPO, (1) basal and food-stimulated serum gastrin concentrations, (2) the concentration of tissue gastrin, and (3) gastrin-producing (G) cell density in the antral mucosa.
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An enzyme-linked immunosorbent assay (ELISA) using monoclonal and polyclonal antibodies against t-PA was used to measure the concentration of tissue-type plasminogen activator (t-PA) in plasma from 34 healthy donors and 92 breast cancer patients with a varying extent of disease.
The concentrations of tissue Ca were very high under biochar with N class in both soils over both growing cycles (Additional file 2: Table 4S, 5S).
The MSW compost and the MIXTURE treatments resulted in statistically equivalent concentrations of tissue P as the NPK treatment in both crops and both years.
Protein concentrations of tissue lysates were determined by DC Protein assay (BioRad) with albumin standards.
The protein concentrations of tissue homogenates were measured according to the Bio-Rad protein analysis protocol Bio-Rad Laboratoriess, Hercules, CA) and Perkin Elmer Bio Assay Reader (Norwalk, CT).
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