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The merger would increase the concentration of the cellular industry in the United States to 3,320, by the HHI scale, up from 2,744 before.
For all experiments, we report metabolite concentrations normalised both with respect to the protein concentration of the cellular debris pellet, and internally (probabilistic quotient normalization, giving relative concentrations).
We sampled cell suspensions from the fermentor under pressure, meaning that we could not exactly control the volume sampled, and so we measured the protein concentration of the cellular debris pellet after extraction in order to assess the amount of biomass sampled, following the method of Villas-Boas et al. [16].
Protein concentration of the cellular homogenate was determined by Bradford assay (Bio-Rad, Hercules, USA).
As expected, the concentration of the cellular dTTP in cells grown under folate deficiency for 15 30 days was significantly reduced compared to the concentration in same cells grown in a normal medium (Appendix Fig S2).
We have shown here that doxorubicin treatment of the oestrogen receptor negative-human breast cancer cell line, MDA-MB-231, in vitro resulted in a marked decrease in the rate of hyperpolarised C label exchange between [1-C]pyruvate and lactate and that this was correlated with a decrease in the concentration of the cellular NAD(H) coenzyme pool.
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We are unaware of any reports of the effect of glucose concentration on the cellular action of clinical TZDs in any in vitro setting.
Using the high-performance liquid chromatography (HPLC) method, the concentrations of the cellular dNTPs were measured.
The protein concentrations of the cellular protein extracts were determined with Coomassie Protein Assay Reagent.
Additional file 1, Table S3 and 4 list the parameter values and the initial concentrations of the cellular signaling molecules.
Protein concentrations of the cellular extracts were measured using a Bio-Rad protein assay kit (Hercules, CA, USA).
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