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The graph related the CL intensity vs. the concentration of testing drug solutions was plotted at 9 experimental points.
In this study, the 3 levels (high, middle and low level) of tritium concentration of testing samples for the loss rate test were prepared similar to the concentrations reported in ASTM D4107.
Similar(58)
MIC (minimal inhibitory concentration) of tested sample against these microorganisms was determined as previous reports [24].
Concentration of tested sample corresponded to the extract obtained from 10 mg dry weight of B. brizantha per mL.
Concentrations of tested compounds were increased from 0.01 nM to 1 μM.
These effects were associated to IIQ-mediated impairment of tubulin polymerization at pharmacologically significant concentrations of tested compounds.
Concentrations of tested samples corresponded to the extracts obtained from 0.3, 1, 3, 10, 30, 100 and 300 mg dry weight of B. brizantha per mL.
The cell culture media containing different concentrations of tested extracts and dead cells was decanted, and viable attached cells into the tissue culture plate were leaved.
These concentrations of tested compounds were used in further experiments.
The concentrations of tested compounds were assumed to be below the Km values of relevant enzymes.
Finally, the cells were treated with different concentrations of tested compounds for further 24 hours.
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