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The molar concentration of test compound producing 50% inhibition (IC50) were calculated for each test compound in a minimum of triplicate and geometric means and 95% CI were subsequently calculated.
Given the small concentration of test subjects, we are pleased with the accuracy for the tested range, but there is significant room for improvement in accuracy for more body types.
Separation factors and efficiencies of separation were evaluated and found to be dependent on (a) time of swelling; in some cases times were deliberately kept short and final equilibrium swelling was not reached, (b) concentration of test solution and (c) mass of test solution relative to that of the initial gel.
The highest concentration of test substances was transferred to the first row of the plate.
A decrease in ergosterol biosynthesis with the increasing concentration of test agents was observed.
The IC50 (the concentration of test compounds required to kill 50% of cell population) was determined (Table 1, Fig. 3).
Decrease in colony forming units with the increasing concentration of test compounds is shown in Fig. 2.
The MIC values were tabulated in terms of percentage concentration (The concentration of test solutions in column 1 was considered as 100%%) (Table 1).
Data generated were used to plot a dose response curve of which the concentration of test compounds required to kill 50% of cell population (IC50) was determined.
The plates were read after 48 h and MIC was determined as the lowest concentration of test agents inhibiting the visible growth.
Similar(1)
The turbidity of all suspensions was standardized for each bacterial strain using a spectrophotometer at 580 nm (Biochrom WPA CO7500 Colorwave Colorimeter), to provide the concentration of test-microorganisms approximately equal to 1 × 10 colony-forming unit/ml (CFU/ml).
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