Sentence examples for concentration of templates from inspiring English sources

The concentration of templates was determined by qPCR with Library Quantification Kits from KAPA biosystems.

The AtTUBRTF and AtTUBRTR (primers for Arabidopsis tubulin gene in Table  3) were used to verify equal concentration of templates for the expression analysis.

Due to high concentration of templates and possibility of effects of template abundance, the reassociated DNA was diluted 1000 or 100,000 times, then amplified using PCR with the CT or TT primers.

Ten 10-fold serial dilutions of P1 with concentrations from 10to1010 copies/μL were used as templates to run SYBR I qPCR The results showed that even at the low concentration of 10 copies/μL, fluorescent signal could still be detected, indicating a high sensitivity and the concentration of templates correlates well with Ct values (Fig.  4).

The effects of CdTe QDs on PCR did not alter by increasing the concentration of template or primers, but were completely reversed by increasing the concentration of Taq polymerase in the PCR mixture.

By varying the concentration of template with respect to titania and niobium precursors, we were able to produce niobium-doped titanium oxides with surface areas of 75 115 m2/g and sufficient electrical conductance to function as fuel cell catalyst supports.

The optimal concentration of template used was determined empirically (Figure S5).

Amplification efficiency, the fraction of template that gets replicated per PCR cycle, was measured by using 10-fold serial dilutions of a positive control PCR template and plotting CT as a function of log [13] concentration of template.

The higher incidence of PCR recombination in the amplicon templates is consistent with previous findings of increased rates of PCR recombination with increased concentration of template DNA [42], [43].

Cycle numbers of the logarithmic linear phase were plotted against the logarithm of the concentration of template DNA to evaluate the number of yeast cells present in each tissue sample homogenate.

PCR amplification of probe fragments used in this study was performed with Taq DNA-polymerase (Promega) with the manufacturer's suggested concentration of template and primers in an Eppendorf Mastercycler gradient system.