The molar concentration of each species was calculated by multiplying the fractional contribution of an individual band by the total concentration of substrate.
It is very large when the initial concentration of substrate is relatively low or high at a constant dilution rate.
At a given concentration of substrate, the amount of products rapidly reached a plateau with the increasing concentrations of enzyme.
The effect of the H2 pressure, the concentration of substrate, solvent and recyclability of the catalyst were studied.
In contrast, a high yield of target product to substrate could be obtained by decreasing the initial concentration of substrate in feed.
For instance, a high density cell cultivation should be carried out by increasing the initial concentration of substrate in feed at a constant dilution rate.
The concentration of substrate that results in 50% of steady-state response (EC50) was 0.7 mM and sensitivity (S) was 3.8 mA/M.
The results showed increased concentration of substrate and intermediate compounds in waster could create more foaming tendency but not effect on foaming stability.
Kinetic rates were affected by initial concentration of substrate and isolates showed distinct growth rates at different 2,4-D concentrations.
Haldane-type kinetic expressions that include the oxygen limitation, the inhibition effect due to high concentration of substrate, and the cross-inhibition between substrates have been considered.
Equation 1: where v is initial velocity of reaction; [E] is total enzyme concentration; kcat is the turnover constant; [S] is the substrate concentration; K0.5 is the concentration of substrate in which enzyme reaches half of maximal velocity and nH is the Hill coefficient31.
