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The presence of transgressive segregants exhibiting higher concentration of shoot K+ and lower NaK ratio than Pokkali suggests the presence of positive alleles in both parents for selective cation transport during salt stress (Fig. 1).
At physiological and ionomic levels, models responded to salinity in a similar way to crop species, and changes in the concentration of shoot Cl− correlated well with tolerance.
The relative Na+ exclusion was calculated by the formula: relative Na+ exclusion = (Na+ concentration of solution × Volume of solution)/(Na+ concentration of shoot × DW of shoot).
However, by comparing the magnitude of the tolerance to Ni, Co, Zn, and Cd resistance conferred by heterologous expression of TgSAT m with the concentration of shoot GSH, across lines with different levels of SAT expression, we established that the effectiveness of GSH in enhancing metal tolerance varies with the metal.
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However, the GSH concentration of shoots with engineered CBSB1-GCSGS increased to 0.7 μM g−1 fresh weight.
In inert substrate, nitrate concentration of shoots was 8-fold greater in Erianthus species than Saccharum species and Saccharum-Erianthus hybrids (Figure 4A).
The Fe concentration of shoots in both OE lines was significantly reduced compared to wild-type under both medium and low levels of Fe supply.
Ion concentrations of shoot and root tissue at the end of the experiment on surviving plants were also affected by salinity levels and varied among species.
Interestingly, the concentrations of shoot K+ in the stressed plants were higher than those of the control plants, perhaps suggesting that rice attempts to maintain as high a K+ concentration as possible during salt stress.
To establish the extent to which Na and K concentrations of shoot cell sap change in response to differences in groundwater salinity.
To evaluate the effect of 2,4-D concentration on shoot regeneration, two concentrations of 2,4-D in the pre-culture medium were tested.
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