Collected oocytes were inseminated by exposure to fixed concentration of partner's treated sperm sample or by intracytoplasmic sperm injection (ICSI).
In addition, hidden or cryptic integrin tail-binding sites in partners can be exposed by mechanical force, proteolysis, or phosphorylation, and the local concentration of binding partners can be regulated by membrane or other subcellular localization signals.
The apparent affinity (avidity) increases in the proximity of the adhesion, probably due to the high concentration of appropriate partners for binding in these regions.
4), Different sides of the adhesions show different dynamics, probably due to a different affinity for paxillin or concentration of the partners with which paxillin interacts.
This buffer effect suggests that the association constant and the concentration of the partners can be tuned by selection so as to make the system robust against halving the input of one of its components.
Increased concentration of the partner protein correlated with the elevated levels of BCA2 protein.
A novel kinetic model is advanced to relate surface plasmon resonance (SPR) data with actual concentrations of interacting partners.
Their differences define the concentrations of binding partners for effectors whose binding site phosphorylations are described in different layers.
(g) Assign initial conditions guaranteeing x i > 0 (prevents division by zero) and x i ≥ x i b ∀ i (prevents negative concentrations of binding partners).
HDM2 is itself a p53 target gene and hence acts as part of a negative feedback loop which maintains low cellular concentrations of both partners under non-stressed conditions (Picksley and Lane, 1993; Wu et al., 1993; Freedman et al., 1999; Michael and Oren, 2003; Bond et al., 2005).
The significance of this study is that we could demonstrate that the increased concentration of the reaction partners due to an active site of the catalyst allows reactions which otherwise would be unlikely.
