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The protein concentration of each sample was determined with a Micro BCA Protein Assay Kit (ThermoScientific).
The concentration of each sample was determined with UV spectroscopy by using the standard curve method (λmax, 484 nm).
Finally, absorbance values were calculated for standards and samples and the concentration of each sample was determined using the standard curve.
The DNA concentration of each sample was quantified using Qubit dsDNA BR Kit according to the manufacturer's instructions (Thermo Fisher Scientific).
The concentration of each sample is 0.1 wt%.
The chelating activity was correlated well with the increasing concentration of each sample.
It was found that the reducing power increased with concentration of each sample.
The protein concentration of each sample was calculated according to a standard dilution series.
The protein concentration of each sample was determined using a bicinchoninic acid assay (Pierce).
The Bradford method was used to quantify the protein concentration of each sample.
Colon cytokines were normalized to total protein concentration of each sample.
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