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A plot of sequence conservation on the surface of the Bub1 Bub3 complex shows an extreme concentration of conserved residues at the interface with the Spc105/Knl1 peptide.
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Examining the MSA of the entire order reveals two regions with high concentrations of conserved CICPs at ∼382 426 and ∼447 522 (Fig. 3, 4C).
The frequent appearance of these saturating response curves in biology arises because many in vivo biochemical reactions are rate-limited by the concentration of some conserved macromolecule (such as DNA or an enzyme).
Otherwise, different initial conditions change the total concentration of a conserved chemical species in the system and lead to lines of fixed points where only a single fixed point is expected.
Moiety-conserved cycles require special consideration in GSDA as the total concentration of the members of the conserved cycle must remain constant.
Figure 3: Quantification of conserved features.
(b) Detailed view of conserved pocket 1.
Alignment demonstrates the position of conserved inhibitory domains.
Supplementary Figure 7 Comparison of conserved regions assessed with CDTS and GERP.
Asterisks represent site of conserved cysteine (C) residues.
This problem is alleviated by a staggered arrangement of conserved variables.
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