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A working stock of all native standards was prepared at 5 mg/L in pure methanol and diluted as required to obtain the desired concentration of calibration standards.
Calibration was carried out by performing a four-parameter fit (absorbance vs nominal concentration of calibration samples, including '0' standard).
The platinum concentration of calibration standards was chosen to cover the expected range of platinum concentrations in the diluted plasma samples: 1, 5, and 10 μg/L.
For measurements of the concentration of calibration beads, a modified impedance counter with an integrated balance for mass determination (22) and an optical flow cytometer, equipped with a motor driven, gravimetrically calibrated 1-mL syringe were used.
Equation (1) reflects the advantage that for relative concentration measurements, the determination of the volume and hence the dilution factor is not required, as both, the cell concentration and the concentration of calibration beads is changed accordingly.
Similar(55)
The concentrations of calibration standards were back calculated to obtain the accuracy of each calibration point.
It has low concentrations of calibration ranges compared to the other existing photometric methods.
Similar catalyst systems have been used previously to confirm concentrations of calibration gases with high accuracy (Baasandorj et al. 2015; Veres et al. 2010).
Briefly, 11 concentrations of calibration solutions (5 500 ng/ml) were prepared fresh every day by spiking CVA into human plasma samples.
Concentrations of calibration standards were chosen to cover the expected range of metabolite As concentrations in blood samples.
To construct an accuracy profile the SFSTP recommend that 3 5 different concentrations of calibration standards and 3 different concentrations of VS (representing high, medium and low points on the calibration curve) are run in triplicate on 3 separate days, (Feinberg et al, 2004; Feinberg, 2007; Rozet et al, 2007).
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