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The protein concentration of ACC synthase was shown to peak at véraison in Nebbiolo Lampia berries [ 17].
Variations in the concentration of ACC, the direct precursor of ET, were observed in infected Chinese cabbage roots during the initiation of secondary infection (Devos et al. 2005).
To determine the appropriate concentration of ACC for aerenchyma and growth studies, its effect on root elongation was examined at concentrations of 1 or 10 µM (data not shown).
We found that in wild type plants, a relatively high (1 μM) concentration of ACC caused a significant increase in short lateral roots, but a non-significant increase in longer lateral roots, opposite to the effect in Arabidopsis, suggesting different optimum levels of ethylene between Medicago and Arabidopsis.
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Bulk FTIR did not reveal the presence of organic molecules in the granules but we hoped that spatially-resolved analyses of the inherently heterogeneous granules would reveal areas of higher, detectable, concentrations of ACC and associated organic functional groups.
The phenylisothiocyanate-derivatized ACC (PTC-ACC) yielded a collision induced dissociation peak of 136 m/ z at 15.2 min (derived from the 237 m/ z M+H parent ion) that was utilized to evaluate relative concentrations of ACC.
Similar results were observed in canola: when a plant-growth promoting Methylobacterium containing ACC deaminase was inoculated into canola roots, it also reduced the concentrations of ACC and ethylene in the plant, increasing root length [ 65, 66].
Differential effects of ethylene on lateral root initiation and emergence have been observed in Arabidopsis, where high concentrations of ACC inhibited the initiation of lateral root primordia but promoted their elongation.
While the concentration of free ACC was comparable after 24 h and 72 h of exposure to Cd, the conjugated ACC content continued to increase towards the later time point.
Exposure to 10 μM Cd on the other hand increased the concentration of both forms of ACC.
To investigate the relationship between SCD1, ACC and lipogenesis in cancer cell proliferation, we treated H460 cells with CP-640186, a specific inhibitor of ACC, at a concentration of 20 µM which inhibits more than 95% of enzyme activity [14].
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