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Removal of rRNA from total RNA was performed using RiboMinus Plant kit for RNA seq (Invitrogen cat. no. A10838-08) using the standard procedure and then concentrated by RiboMinus concentration module (cat no. K155005), according to the manufacturer's instructions.
rRNA-depleted total RNA was concentrated using the Ribominus concentration module (Life Technologies, CA) and assessed on the Agilent 2100 Bioanalyzer for the confirmation of rRNA removal.
Large ribosomal RNA molecules were selectively depleted from the total RNA using a RiboMinus™ Eukaryote Kit for RNA-Seq (Life Technologies, CA, USA), and mRNA was concentrated and purified on a RiboMinus™ Concentration Module (Life Technologies, CA, USA).
The thermal output of the concentration module almost doubles the reference one and the electrical power registered is more than 4.5 times in the case of the concentrating configuration.
RT PCR data were analysed using the comparative concentration module of the Rotor-Gene software, which is based on 46.
After incubation the RNA was purified using the RiboMinus Concentration Module (Invitrogen), and the size and yield of RNA was determined using the Agilent bio-analyzer PICO chip.
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The manufacturer's manual was followed but the RNA was co-precipitated with 1 μl glycogen, using 2.5 volumes 100% ethanol and 0.1 volumes 3 M sodium acetate, instead of the concentration modules included.
In concentrated systems, employing modules, concentration polarisation and module pressure drops will also affect overall process performance.
Detection capability was determined in a series of comprehensive experiments with systematic changes in feed concentration and module operating parameters including upstream pressure, flow rate, and feed composition.
Concentration field module describes the concentration of target chemical in each location.
It can therefore be used for the design and optimisation of high concentration photovoltaic modules.
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