Sentence examples for concentration forward from inspiring English sources

In the UK, media attention has focused more on Johnson's observations that modern computer games require concentration, forward planning, lateral thinking and sustained problem solving - and, as such, offer a "cognitive workout" that can benefit overall mental development.

Other assay conditions including MgCl2 concentration, forward primer, reverse primer, and probe concentrations were optimized for each probe primer set using total RNA.

All reactions also contained 900 nM final concentration forward and reverse primers, 200 nM Probes (see Additional file 6 for primer and probe sequences and Additional file 7 for details of assay performance) and 5 ng template DNA.

All reactions also contained 2 × DA sample loading reagent, 900 nM final concentration forward and reverse primers, 200 nM Probes (see Additional file 2 for details of primer and probe design, Additional file 6 for primer and probe sequences, and Additional file 9 for assay performance) and 5 ng DNA.

A 30 µl reaction mixture was assembled consisting of second round cDNA diluted 1 1000, dNTP mix (0.2 m m in final concentration), forward and reverse primers (0.2 µM each in final concentration), Platinum Taq PCR buffer, 1.5 m m MgCl2 and 1.8 U of Platinum Taq DNA polymerase (Invitrogen life technologies, Carlsbad, CA, USA).

The PCR mixture contained 12 μL of 2× Brilliant QPCR Master Mixture (Stratagene), 2.5 μL of 100 nmol/L (final concentration) TaqMan probe, 2 μL of 200 nmol/L (final concentration) forward and reverse primers, and 5 μL of template DNA.

Wells (20 µl final volume) contained 10 µl of iQ™ SYBR Green Supermix (Bio-Rad), 500 nM concentration of forward and reverse primers and 5 µl of cDNA.

In univariate analysis only gender and CSF chloride concentration went forward to entry into the final model, but neither remained significant.

The reactions (20 µl final volume) contained 10 µl of SYBR GreenER qPCR SuperMix (Invitrogen), 500 nM concentration of forward and reverse primers and 5 µl of cDNA.

PCR reaction: 150 nM final concentration of forward and reverse primers, 6 µl of QuantiTect SYBR Green PCR Master Mix (Qiagen, Valencia, CA) and 50 ng of cDNA template in a total of 12 µl.

In all PCR reactions, the concentration of forward and reverse primer was 250 nM each.