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By increasing the initial ion concentration, final size of metal nanoparticles increase [49].
Induction was carried out at OD600 1.0 with 1/10 normal inducer concentration (final concentration of 20 ng/ml).
The buffers used were glycine/HCl (0.2 M final concentration, final pH 2.0, 2.6 or 3.0), citrate/NaOH (50 mM final concentration, final pH 3.5, 4.0, 4.5.05.0 or 5.5) and MES buffer (50 mM final concentration, final pH 6.0, 6.5 and 7.0).
Finally, 250 μl of either DMEM alone, or DMEM containing 4 × final concentration (final concentration was 20 ng/ml) of PMA (Sigma, UK) was added to the cells.
At the end of the aerobic growth phase (when the glucose was consumed) SH or SSL was added to the culture to a final concentration of 25% of the initial concentration (final volume ratio in the culture 1 4).
The recombinant bacteria were inoculated in LB medium and cultured at 37°C to OD600 of 0.4 to 0.6, with different IPTG concentration (final concentrations of 0.1, 0.2, 0.6, 0.8, and 1.0 mmol/L) to induce expression, and induced for 4 h under the determined optimal concentration of IPTG.
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24 hours later, add nocodazole to the culture in 6 concentrations (final concentrations; 0, 2.5, 5, 7.5, 10, 12.5 ng/ml).
The cells were then treated with 50 μL of different concentrations (final concentrations 10, 25, 50, and 100 mg/L) of tested substances.
Imatinib was added to the media in increasing concentrations (final concentration: 0, 1, 3, 5, 10, and 20 mM) across the plate and cells incubated for 60 hr.
Compliance was confirmed by a marked increase in plasma quercetin concentrations (final concentrations of both trials, 0·3 0·45 µmol/l) and also in the monomethylated derivatives isorhamnetin and tamarixetin.
This equation states that the initial volume × initial concentration = final volume × final concentration.
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