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Additionally, melanin content of each individual line was determined spectrophotometrically and the concentration extrapolated from a standard curve (Fig 5A).
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Based on these values, the following concentrations, extrapolated from human doses, were used: HC at a dose of 5 mg/kg/day [ 52]; MNZ at a dose of 10 mg/kg/day [ 32].
Each sample was assayed in duplicate and the concentration was extrapolated from the included standards.
Siderophores concentration was extrapolated from a standard curve and represented in mM of desferrioxamine B (DFOB).
Sample concentration was extrapolated from the lineal regression equation of the standard curve used and results are shown in fg/ μL ± SEM.
Actual concentrations were extrapolated from a standard curve of known concentrations (0 10 mmol/l L-alanine).
Final Ang-1 and Tie-2 concentrations were extrapolated from standards curves and normalized to total protein concentration.
Using the OD (405 nm) absorbance values, hormone concentrations were extrapolated from a standard curve that related the absorbance to concentration of either the IAA or ABA standard.
Inhibitory concentrations were extrapolated from concentration effect curves after linear regression analysis.
A standard curve was generated from each kit and the absolute concentrations were extrapolated from the standard curve.
Total T4 concentrations were extrapolated from a standard curve generated using six T4 standards ranging from 0 to 20 µg/dL.
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