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The first part of the study consists of sonication (572 kHz) in the presence of zero-valent iron (ZVI) with or without H2O2 to select and optimize the operational parameters as frequency, time, initial solute concentration, dose of reagents and pH.
In the batch adsorption experiments, the effect of different parameters (i.e., pH, contact time, initial CR concentration, dose of biosorbent, and temperature) on adsorption of CR was studied.
The cytotoxicity started at a concentration dose of 0.1% when HepG2 cells were treated and incubated up to 72 hr.
Based on these analyses, it was estimated that the concentration (dose) of 267 in the 267/Dt combination required to achieve an ED50 could be reduced by up to 3.6-fold in the LCC6 cell line.
Further endpoint analysis in cell lines with low Her2 levels revealed that the 267/Dt combinations resulted in: a three-fold decrease in concentration (dose) of 267 required to achieve 50% inhibition of P-AKT; and a dramatic disruption of normal filamentous-actin cellular architecture.
Group III animals received vitamin E at a concentration of 50 mg/kg body weight and animals of groups IV, V and VI were given extract of Podophyllum hexandrum at concentration dose of 20, 30 and 50 mg/kg body weight.
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In all three concentration doses of resistin, pretreatment with 1 ng/mL of AICAR inhibited the resistin effects on SK-Hep1 cell adhesions to HUVECs, compared to treatment with resistin-only cells.
The article provides information on the impact of contact time, initial Cd II), Co II), Zn II), and Pb II) ion concentrations, dose of the sorbents, solution pH and temperature on the adsorption capacity.
At the lowest concentration dose response of 0.025 mg/ml, only ethanol extract exhibited the highest percentage inhibition of 89.35 ± 7.07 with IC50 value of 0.023 mg/ml.
Based on this consideration, we investigated the efficacy of low-concentration doses of metformin alone or in combination with tamoxifen in a model of ER-positive breast cancer that reflects treatment strategies in common clinical use.
To better understand the various pathways associated with taxane resistance, our laboratory selected MCF-7 breast tumor cells for survival in increasing concentrations (doses) of paclitaxel (MCF-7TAX-1 cells) [ 24] or docetaxel (MCF-7TXT cells) [ 23].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com