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Unbound biotin was removed via a PD-10 column (GE Healthcare) and the resulting biotinylated protein was concentrated in an Amicon-30 concentration device.
All clinical samples were concentrated and enriched by a virus concentration device to increase the virus load.
The GS-containing fractions were pooled and dialyzed against 10 mM Hepes pH 7.4 and concentrated to 5 mg.mL-1 on a centrifugal concentration device with a 10 kDa MWCO membrane (VIVASCIENCE).
The purest fractions were pooled and concentrated to 500 μl using an Amicon Ultra 4 concentration device (Millipore Corporation, Bedford, MA, USA) with a 100 kDa cut-off.
If the protein concentration was less than 3 mg/mL after combining peak fractions, proteins were denatured by dialyzing into Buffer D with 6 M urea at 0.1 M KCl, and then concentrated at 4°C to approximately 3 mg/mL using a Centricon-10 concentration device (Millipore Corporation).
The teetered bed separator (TBS) is a gravity concentration device which operates on the principle of hindered settling.
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For crystallization trials, purified SitA was concentrated to >150 mg.ml−1 using centrifugal concentration devices with 10 kDa molecular weight cut-off membranes (Amicon ultrafree, Millipore).
High reflective mirrors are commonly used in solar concentration devices.
The focal length is critical for the design of micro sample concentration devices.
They have developed various biomolecule and cell concentration devices using this phenoemenon, enabling higher detection sensitivities for immuoassays, enzyme activity assays, and cell based assays.
Valeo has adopted a magnetic technology and has improved the performance by adding specially designed flux concentration devices.
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