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In order to maintain maximal ethanol production, nutrient limitations were counter-steered by proportionate supplementation of a 100× nutrient concentrate when the nitrate concentration was below 50% of the BG11 concentration (determined with Quantofix Nitrate/Nitrite, Macherey-Nagel, Düren, Germany).
The D-limonene concentration determined with high-performance liquid chromatography (HPLC) for fresh, dried, and pretreated biomass was 0.76%, 0.32%, and 0.09% (v/w), respectively.
Suspended solids were determined by gravimetry, water temperature with mercury thermometer, water transparency with a Secchi disk, and photosynthetic pigments were extracted in acetone (90%) for 12 h and the concentration determined with a spectrophotometer.
Light and heavy isotope labeled RAW264.7 cells were lysed with 8 M Urea, and then centrifuged at 12000 g for 20 min. Protein concentration, determined with the BCA assay, was adjusted to 1 mg/ml.
MBCstat: minimal bactericidal concentration determined with stationary-phase bacteria.
Suspensions were filtered through cheesecloth and the spore concentration determined with a hemacytometer.
Similar(49)
The mycoproteins were extracted with ultrasonication and their concentrations determined with Coomassie Brilliant Blue.
Aspergillus niger cell-free extracts were prepared as described by Kobayashiet al. (2013), and the protein concentrations determined with a Bradford Protein Assay Kit.
In contrast, the concentrations determined with the phantom replacement method were clearly erroneous when in vivo coil loading conditions were not properly replicated during the calibration session.
Blood glucose concentrations determined with bedside point-of-care glucometers provides inaccurate measurements in critically ill patients.
The cell disruption kit was supplemented with phenylmethanesulfonyl fluoride (PMSF), a protease inhibitor, and protein concentrations determined with a Bradford Protein Assay Kit (Beyotime, China).
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