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Carbon dioxide gas concentration determination using infrared gas sensors combined with Bayesian regularizing neural networks is presented in this work.
Of this suspension, 100 μl was taken for protein concentration determination using the BCA assay.
All samples were processed for melting curve analysis, amplification efficiency and DNA concentration determination using LightCycler® 2.0 System.
Sample was stored at -80°C for minimum of 24 hours before concentration determination using a modified Bradford assay [ 13].
An aliquot was set aside for protein concentration determination using the Precision Red Advanced Protein Assay Reagent supplied with the kit.
After protein concentration determination using the Bradford assay (Bio-Rad, Hercules, CA, USA), equal amounts of protein from all samples were treated or mock-treated with Endoglycosidase H Rochee, Indianapolis, IN, USA) according to the manufacturer's instructions.
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The resistance level was further evaluated by Minimal Inhibitory Concentration (MIC) determination using the microdilution method (Figure 3B).
To achieve this, liquid - liquid extraction (LLE) was employed for purification and concentration followed by direct determination using LC-MS/MS.
Antimicrobial activity was estimated by minimum inhibitory concentration (MIC) determinations using a broth microdilution procedure basically according to a guideline of the Clinical Laboratory Standards Institute (14).
An extracellular concentration of H2O2was quantified by amperometric determination using the Apollo4000 (Worldprecision Instruments) with the H2O2sensor ISO-HPO-2 sensor tip according to the manufacturer's instruction.
The actual HC concentration is 180 nM based on our determination using enzyme-linked immunosorbent assay.
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