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At a critical concentration, complementary microparticles form an aggregate that sinks to the bottom of the cuvette and the optical density falls to zero.
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Our results revealed a satisfied linear correlation between the peak current of differential pulse voltammetry and the concentration of complementary target DNA.
The peak current (ipa) of PZH is linearly proportional to the logarithmic concentration of complementary target DNA in the range from 2.0 × 10−9 mol L−1 to 5.0 × 10−7 mol L−1 with the detection limit of 3.8 × 10−10 mol L−1.
The results indicated that in pH 7.0 Tris HCl buffer solution, the oxidation peak current was linear with the concentration of complementary strand in the range of 6.2 × 10−8 3.1 × 10−7 mol/l with a detection limit of 5.3 × 10−9 mol/l.
The peak currents of adriamycin were linear with the concentration of complementary DNA, which ranged from 1.0 × 10−8 to 1.0 × 10−13 mol dm−3 with a detection limit of 3.5 × 10−14 mol dm−3 (signal/noise ratio of 3) under the optimal conditions.
We conducted further studies of hybridization time and temperature using the optimum concentration of complementary DNA.
These regions benefit by a concentration of complementary resources that might include leading research universities, low cost or highly trained labor, and geographic bounty.
Table 3 can give us an idea about how Ids and Vgmin parameters change with different concentration of complementary DNA molecules which reveals the sensitivity of Vg,min towards the hybridization of the target DNAs.
The linear regression equation was y = 1.972 log x + 21.5996 with a correlation coefficient value of 0.996 (x is the concentration of complementary sequence, M; y the peak current of DNR, μA).
Plates were diluted to 1 μM working concentration in complementary 1× siRNA buffer in a 96-well plate format.
In the buffer with suitable Na+ concentration, the complementary fusion curve and the SM curve from the same group demonstrated apparent "branching" effects.
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