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Critical parameters (concentration, buffer, pH, voltage) assessed effects on resolution, analysis time and efficiencies.
For that purpose, the gradient, the mobile phase composition (buffer concentration, buffer pH, and elution salt), and the temperature were optimized.
The MCs (buffer, surfactant cosurfactant, oil) and the PVs (voltage, buffer concentration, buffer pH) were simultaneously changed according to a MPV experimental design.
Therefore, a central composite design was used and the following factors were investigated and varied simultaneously: buffer concentration, buffer pH and dimethyl-β-CD concentration.
Seven chemical and physical parameters namely pH, cisplatin concentration, buffer concentration, deposition potential, square wave frequency, amplitude of the pulse and step potential have been optimized.
The effect of lipid (phosphatidylcholine and cholesterol), enzyme concentration, buffer pH, type and time of sonication, and sucrose concentration were the parameters selected for this study.
Similar(35)
Thus, sample fixation (e.g. formaldehyde concentration, buffered or non-buffered formalin, duration of fixation, tissue size and sample segmentation) should be carefully considered.
Once a stable current was observed, the synthetic medium was switched to the desired PDWW concentration buffered with 50 mM KH2PO4, pH 7.0 (Jiang et al. 2009), and MFCs were operated in continuous mode for 60 days.
Beads were washed with increasing salt concentration buffers, chromatin eluted by SDS-bicarbonate buffer and samples were decrosslinked at 65°C in presence of high salt concentration.
(A and B ) Whole plant protein extracts were analyzed in in-gel protein kinase assays with the free Ca2+ concentration buffered to either 150 nM or 400 nM.
Chlorophylls and carotenoids were extracted in acetone (80% final concentration buffered with Na2CO3) and measured by fitting of the absorption spectrum of acetone extracts [ 79].
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