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The quality of the total RNA RNA Integrity Numberr > 6.5 and 28S:18S > 1.0) was verified using a 2100 Bioanalyzer RNA Nano chip device (Agilent, Santa Clara, CA, USA) and its concentration ascertained using an ND-1000 spectrophotometer (NanoDrop, Wilmington, DE).
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We use maternal blood lead concentrations ascertained during study visits scheduled within each trimester.
The axial and radial profiles of the solid concentration were ascertained by sampling the suspension.
DNA purity and concentration was ascertained by use of a Nanodrop™ 1000 spectrophotometer.
Before each experiment, eGFP-CaM was thawed and centrifuged for 10 min at relative centrifugal force 20,800 × g and its concentration was ascertained using FCS.
The lysate concentration was ascertained and was equally diluted in immunoprecipitation dilution buffer (0.01% SDS, 1.1% Triton X-100, 1.2 mmol EDTA, 16.7 mmol Tris HCl, 167 mmol NaCl, pH 8.1).
The cell concentrations were ascertained by direct counting using Trypan blue.
A significant positive relationships between baseline values of plasma ADM and plasma NA concentrations were ascertained for older and younger groups of subjects (r = 0.76, P < 0.001; r = 0.62, P < 0.01; respectively).
Therefore, we looked at the production of 2-chloroethanol versus Pi concentration to ascertain the changes occurring in the overall yields of chloroethylating electrophiles.
The diagnostic concentrations to ascertain genotype frequencies were determined from the concentration-mortality curve of the F2 generation.
The concentration at which resistant mutants did not emerge (the mutant prevention concentration) was also ascertained.
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