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Using blood pressure, heart rate and other indices of anesthetic depth, adjust the inhaled agent concentration as needed (or give increments of IV agents such as fentanyl or propofol).
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Subjects were medicated with a pre-anesthetic mix of Telazol (tiletamine/zolazepam) 4.4 mg/kg and xylazine 2.0 mg/kg intramuscularly, endotracheally intubated and anesthetized with 3% inhalational isoflurane (with concentration adjusted as needed to maintain anesthesia).
The final cell concentration in the suspension was adjusted as needed to achieve the desired cell density in the experiments by the addition of 1 mL of cell suspension.
Similarly, for determining the acute toxicity and protective efficacy, the detergents and CHX were also delivered in PBS but at various concentrations as needed.
as needed.
Add hygromycin to a final concentration of 100 μg/mL as needed.
Patients were permitted to use immediate-release, opioid-based, oral pain medications such as oxycodone hydrochloride oral solution (1 mg/mL concentration) in the clinic as needed for relief of treatment-related discomfort.
Strains were grown in a final volume of 50 mL MEMα supplemented with 10 mM pyruvate with freshly prepared FeSO4 added at a final concentration of 40 μM as needed to generate iron-replete growth conditions.
In fluorescence spectroscopic measurement, highly concentrated solutions are not suitable, because fluorescence is very sensitive method and needs low concentration as far as inner filter effect is occurred in which molecules of the solution as a whole is not uniformly exciting and then emitting.
Thus, this issue is addressed by proposing the use of an external sensible heat storage system, added to the regenerative oxidizer, capable of storing the excess of heat released in the oxidizer during rich concentration periods, and using it to heat the feed as needed during lean concentration periods.
Mixtures of antibiotics (AB) were added to media as needed (end-concentrations: ampicillin 100 µg/ml, kanamycin 50 µg/ml, streptomycin 100 µg/ml, tetracycline 20 µg/ml, chloramphenicol 20 µg/ml).
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