Exact(2)
This was followed by filtration using No.1 filter paper (Whatman, USA) before concentrating using a rotary evaporator.
The supernatant was passed through 0.22 μm filter (Millipore) and kept at 4°C before concentrating using stirred cell (Amicon, model number 8200, Millipore) with 300 kDa cut-off membrane (Ultracel PL30000; Millipore) followed by further filtration through a 30 kDa cut-off membrane (Biomax PB 300,000; Millipore).
Similar(58)
The filtrate was concentrated using vacuum concentrator.
The protein solutions were then concentrated using centrifugal concentrators.
The protein and RNA solutions were repeatedly exchanged into ITC buffer and concentrated using centrifugal concentrators.
Fractions were concentrated using Vivaspin centrifugal concentrator (Sartorius).
Viral particles were concentrated using Lenti-X concentrator (Clontech).
The eluted protein was concentrated using Vivaspin 2 Concentrator (Vivascience, Germany).
Crude oligosaccharides from charcoal fractionations were concentrated using a SpeedVac concentrator (SC210P1-115, Thermo Scientific) before SEC fractionation.
DNA was then purified and concentrated using the DNA Clean & Concentrator kit (Zymo Research, Irvine, CA, USA).
The two pools could then be concentrated using Centricon-10 concentrators (Millipore).
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