Exact(1)
Resulting elutant was then dialyzed again in a solution of 0.1X PBS/9 mM HEPES pH 7.4 containing 0.05% Tween-20 (Fisher) before concentrating down to a desired level.
Similar(7)
The pKM101 CC eluted from the StrepTrap HP column (GE Healthcare) was concentrated down to 500 μl at a final concentration between 5 and 10 mg/ml depending on the preparation.
Subsequently each 2 ml protein fraction was dialysed against de-ionised water using Spectra/Por 3 dialysis tubing with a MWCO of 3.5 kDa (Spectrum Laboratories, Inc., Rancho Dominguez, CA, USA) and then concentrated down to 0.5 ml using a vacuum concentrator (Speed-vac SPD131DDA, Thermo Scientific, Basingstoke, UK).
The NA-containing fractions were pooled and concentrated down for further analysis.
Eluate was buffer exchanged into 1× PBS/20% glycerol and concentrated down to 150 µl volume.
This washing protocol was repeated thrice and the resulting wet solid was concentrated down with toluene (10 ml) in a rotary evaporator.
Postsampling, the filters were extracted in 10 mL toluene via sonication and concentrated down to a sample volume of 5 mL.
The plush toys were then extracted in 200 mL of hexane via sonication and concentrated down to a sample volume of 5 mL (Gurunathan et al. 1998).
Related(1)
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