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Huwe1 knockdown in myotubes was performed by incubating lentiviral concentrates in the presence of 8 µg/ml polybrene on differentiation day 4 for two days, followed by puromycin selection for another 2 days (1 µg/ml) to eliminate uninfected myotubes.
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In 2015, Hoffman et al. used a cell-based model to demonstrate improvement of multiple parameters of thrombin generation after treatment with prothrombin complex concentrate in the presence of therapeutic levels of dabigatran [11].
The protein sample was dialysed against a buffer containing 50 mM Tris (pH 8.0) 100 mM NaCl, 20% glycerol, 1 mM DTT, and concentrated in the presence of 8 mM Mega8.
The emulsifying properties of beef lung protein concentrate (BLPC) in the presence of xanthan gum were investigated under steady state conditions using a continuous rotor/stator device.
Copper concentrate dryer means a vessel in which copper concentrates are heated in the presence of air to reduce the moisture content of the material.
5 g of cellulose were added to 5 ml concentrated sulfuric acid in the presence of 0.1 g silica and then steered for 10 min.
Bioleaching assays with the mixotrophic strain Cutipay showed a strong increase in copper recovery from chalcopyrite concentrate at 50°C in the presence of chloride ion, a relevant inhibitory element present in copper bioleaching processes.
The protein was concentrated and crystallized in the presence of a non-hydrolyzable ATP analogue, AMP-PNP, which was easily recognized in the electron density map inside the active site.
Likewise, the dimeric form could be produced by concentrating the protein in the presence of denaturant.
Culture filtrates were concentrated 20-fold in the presence of protease inhibitors as described in Section 2.5.
Total RNA was treated with the RiboMinus Eukaryote Kit for RNA-Seq (Invitrogen, Carlsbad, CA) to remove ribosomal RNA, and the rRNA-depleted RNA was concentrated by ethanol precipitation in the presence of a glycogen carrier (Ambion).
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