Signal pathways that govern cellular senescence have been concentrated on, yielding different and seemingly inconsistent results in different systems.
The EtOH solution was concentrated under vacuum yielding 42 g (10.50 % w/w from dried plant material) of crude ethanol extract of Annona vepretorum (Av-EtOH).
The filtrate was concentrated under reduced pressure, yielding the Ardisia crispa root hexane fraction (ACRH).
The organic layer was dried with anhydrous Na2SO4 and concentrated under reduced pressure yielding 22.3 g of slightly yellow crystal.
After being washed with 10% HCl aqueous solution (4 times, 45 mL each time), the organic phase was dried with anhydrous calcium sulfate and concentrated under reduced pressure yielding a brown oil-like crude product.
Breeding programmes for much of the past half-century have concentrated on high-yielding, pest-resistant, fast-growing crop varieties, which now dominate over half of all the land planted to rice, maize and wheat.
After filtration, the crude extract was concentrated to dryness under vacuum, yielding 72 g of crude extract (14.4% yield).
The EtOAc layer was concentrated using a rotary evaporator yielding tert-butyl 5- 2-azidoacetamido)pentylcarbamate as a brown yellowish oil, which was further purified by column chromatography on silica gel (EtOAC/ n-heptane 1:1).
The proposed ATPS appears to be a promising alternative to conventional first-step operations for direct recovery of collagenase from fermented broths, yielding a concentrate enzyme solution able to effectively hydrolyze collagen.
