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Fission yeast strain (JB795) expressing a C-terminally TAP-tagged version of Cbf11 from its endogenous chromosomal locus was grown to mid-exponential phase in 4× concentrated YES medium (Formedium), washed twice with water, and snap-frozen in liquid nitrogen.
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The results in September suggested that Labour yesses were concentrated in the heartlands, and Friday's poll fits with that.
"Here I would start by pointing out they are taking more of a concentrated risk by having their assets concentrated in their business, which ultimately, yes, they control, but it's a very strong risk position.
In this referendum, working-class areas produced strong no votes and yes votes were concentrated in affluent places.
Only a quarter of the country answers yes when asked if Mr. Bush "has concentrated on problems that matter most to you".
In fact Democrats are working for a "yes" vote, figuring they may win two districts where their voters are concentrated.
Cells were cultured over night in YES containing glucose at 0.5% weight per volume until they reached mid-log phase, concentrated by centrifugation, and visualized using phase microscopy using a Zeiss Axioscop microscope with a Plan NeoFluor 40× objective.
How concentrated?
He concentrated.
I concentrated.
"I was very concentrated.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com