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The PCR products were purified and concentrated with two Qiaquick columns (Qiagen), according to the manufacturer's protocol, and eluted with 30 µl Buffer EB (Qiagen) per column.
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This year I concentrated, with one delightful exception, on biographies and autobiographies.
Consumer banking in Canada is highly concentrated, with five big banks competing for most of the business.
MANAGING SECURITIES The market crash of 2008 has instilled a fear of being overly concentrated with any one manager or firm.
Yet the snafu at the state-controlled firm highlights worrying vulnerabilities at the heart of British banking.The first is that the market is highly concentrated, with just five big consumer banks.
The market would also be highly concentrated, with just three large companies (SSE, a power utility, Standard Life, an insurance company and Royal Bank of Scotland) comprising 40%.This financial focus has weighed on the hypothetical index in recent years.
The database market is highly concentrated with the three main proprietary database vendors – Oracle, IBM and Microsoft – accounting for approximately 85% of the market in terms of revenue, the commission added.
Working backward, Milner suggests that the consumer internet — originally valued at around $350 billion, the majority of which was concentrated with the top five consumer companies about 10 years ago — will make another massive leap in the next 20 years from where it is today.
The beads were concentrated with the MPC, washed three times in transcription buffer, and resuspended in transcription buffer to a final concentration of 10 mg/ml.
Here the three divisions concentrated, with their supplies carried in massed horse-drawn transport and on long camel trains, clogging the roads.
As shown in figure 5, the biotinylated scFv was eluted with high yield and purity, and ten times concentrated with respect to the culture supernatant.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com