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At first glance, a make-up brand and a milliner may seem like unconventional bedfellows, but considering the focus of both is concentrated to the same area – namely, one's head – collaboration doesn't seem that strange.
To assess the ability of lysate derived from previously established cell lines to support PMCA, we prepared cell lysate in which PrPC was concentrated to the same level as wild type mouse brain material (Fig. 1A).
The IPs are, however, directly comparable across mutants, as all were concentrated to the same degree.
The viscosities of both LOCM and IOCM markedly exceed those of plasma, yet the higher osmolalities of LOCM convey a renoprotective effect: by virtue of their greater tubular osmotic force, LOCM cannot be concentrated to the same extent as IOCM.
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MurA and MurG –the first and the last enzyme of this pathway respectively– concentrate to the same locus during exponential growth and are diffuse during stationary growth.
Lesions in specific disorders such as Alzheimer's disease and schizophrenia are located in high degree regions, but not necessarily in the highest degree hubs, and not hub-concentrated to the same extent within different lobes of the cortex.
The protein was then concentrated to 5 mg/ml in the same buffer.
MEL5 was purified and concentrated to ∼150 μM on the same day of surface plasmon resonance analysis to reduce the likelihood of TCR aggregation affecting the results.
Because of saturation of the MALS detector, concentrations of >20 mg/mL could not be analyzed by this technique, but analysis of MDH concentrated to >100 mg/mL using the same column matrix coupled to a UV vis detector indicates the presence of 10% dimer and 90% oligomer.
The agonist was then added alone or with antagonist in MEM for 10 min. Treated and control media were collected and concentrated to 50 μl at the same time and in an identical manner, using Amicon Ultra Centrifugal Devices 5 kDa (Millipore) or Vivaspin 20, 5000 MWCO (Sartorius Biolab Products; MWCO is molecular weight cut-off).
For EPR spectral acquisitions, the proteins were concentrated to >100 μM CYP450 in the same buffer.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com